Nematicidal Effects of Bacillus subtilis and Bacillus pumilus Against Meloidogyne incognita infecting Pea

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Wafaa Mohamed El-Nagdi H. Abd-El-Khair Mona G. Dawood

Abstract

Bacillus subtilis (BS) and B. pumilus (BP1 and BP2) were applied alone as well as in combination for controlling Meloidogyne incognita infecting pea in pot experiment. Single treatments of bacteria significantly reduced the numbers of juvenile (J2) in soil (50-80%) ,J2 in roots (57-78%), females (55-74%), galls  (65-74%) and egg-masses (56-70%) , while the combination treatments of them significantly reduced the same parameters in the ranges of 29-76% , 61-77% , 46-69% , 47-67% and 40-61% , compared to 71 , 70 , 70 ,68 and 65% , with Carbofuran 10%, respectively.  Results showed that BP2 highly reduced the J2 in soil , BS highly reduced J2 in roots BS and BP1 highly reduced the numbers of females, galls and egg-masses in roots. On the other hand, BP1 + BP2 highly reduced the numbers of J2 in soil, J2, females and egg-masses in roots, while BS+BP1+BP2 treatment highly reduced the galls number.  B. subtilis and B. pumilus, as single treatment, in some parameters, had more nematicidal activity against M. incognita, than in combination treatments. Treatments also significantly increased the growth parameters of pea plants shoot length, shoot fresh and dry weight, leaves numbers and pod fresh and dry weight. The treatments improved the soluble protein and total phenolic compounds in treated pea plants.  

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El-Nagdi, W. M., Abd-El-Khair, H., & Dawood, M. G. (2018). Nematicidal Effects of Bacillus subtilis and Bacillus pumilus Against Meloidogyne incognita infecting Pea. Advances in Agricultural Science, 6(4), 52-59. Retrieved from http://aaasjournal.org/submission/index.php/aaas/article/view/92
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References

Abd-Elgawad, M.M.M., 2008. The current status of phytonematode management in Egypt with special reference to applicable nematicides. Egyptian Journal of Agro nematology 6, 33-46.
Abd-El-Khair, H, El-Nagdi, Wafaa, M.A. and Ameen, Hoda, H., 2016. Antagonistic effects of rhizobacteria isolates against Meloidogyne incognita infecting tomato plants under greenhouse conditions. International Journal of PharmTech Research 9(10), 097-107.
Ahmadian, G, Degrassi, G, Venturi, V, Zeigler, D R, Soudi, M and Zanguinejad, P., 2007. Bacillus pumilus SG2 isolated from saline conditions produces and secretes two chitinases. Journal of Applied of Microbiology 103(4), 1081–1089.
Bradford, MM., 1976. A rapid and sensitive method for quantization of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72, 248-254.
Choudhary, D C and Johri, B N., 2009. Interactions of Bacillus spp. and plants – with special reference to induced systemic resistance (ISR). Microbiological Research, 164(5), 493-513.
Dawar, S, Tariq, M and Zaki, M J., 2008. Application of Bacillus species in control of Meloidogyne javanica (Treub) Chitwood on cowpea and mash bean. Pakistan Journal of Botany, 40(1), 439-444.
Dubois, M, Cilles KA, Hamilton J, Rebers R, Smith F., 1956. Colorimetric method of determination of sugars and related substances. Analytical Chemistry 28, 350-356.
El-Nagdi, Wafaa MA and Abd-El-Khair, H., 2017. Application of certain bacterial and fungal species for controlling Meloidogyne incognita parameters in cowpea. International Journal of Entomology and Nematology 3 (2), 70-76.
El-Nagdi, Wafaa MA and Abd-El-Khair, H., 2014. Biological control of Meloidogyne incognita and Fusarium solani in dry common bean in the field. Archives of Phytopathology and Plant Protection 47(4), 388-397.
Gokte, N. and Swarup, G., 1988. On the potential of some bacterial against root-knot and cyst nematodes. Indian Journal of Nematology 18,152-153.
Gowen, SR. and Ahmad, R., 1990. Pasteuria penetrans for control of pathogenic nematodes. Aspects of Applied Biology 24, 25-32.
Hussey, RS and Berker, KR., 1973. A comparison of methods of inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter 57, 1025-1028.
Kloepper, J. and Ryu, C., 2006. Bacterial endophytes as elicitors of induced systemic resistance. In; Microbial root endophytes, Eds. In; Schulz, B.; Boyle, C. and Siebern, T., Springer-Verlag, Heildelberg, 33-51.
Lee, Y S. and Kim, K Y., 2016. Antagonistic potential of Bacillus pumilus L1 against root-knot nematode, Meloidogyne arenaria. Journal of Phytopathology 164 (1), 29–39.
Leifert, C, Li, H, Chidburee, S, Hampson, S,Workman, S, Sigee, D, Epton, HA and Harbour, A. ,1995. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. Journal of Applied Bacteriology 78(2), 97-108.
Moghaddam, M R, Moghaddam, EM, Ravari, S B and Rouhani, H., 2014. The first report of Bacillus pumilus influence against Meloidogyne javanica in Iran. Journal of Crop Protection 4, 3 (1), 105-112.
Mohamedova, M. and Samaliev, H., 2011. Effect of the rhizobacterium Bacillus subtilis on the development of the root-knot nematode Meloidogyne arenaria at different temperatures. Agricultural Science and Technology 3 (4), 378 – 383.
Priest, F., 1993. Systematic and ecology of Bacillus. In: Sonenshein , A.L.; Hoch, J.A. and Losick, R. Bacillus subtilis and Other Gram-Positive Bacteria. 2nd ed. American Society for Microbiology Press.
Richardson, AE, Barea JM, McNeill AM, Prigent-Combaret C., 2009. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil ,321, 305–339.
Samaliev, H. and Stoyanov, D., 2008. Parasitic nematodes of crop plants and their control. Agriculture University Academic Press, Plovdiv 78-122.
Sarangi, T. and Ramakrishnan, S., 2016. Influence of Biomolecules of Bacillus spp. against Phytopathogens: A Review International. Journal of Current Microbiology and Applied Sciences 5 (7),131-134.
Satyandra, S., Bhagawati, B. and Goswami, B.K., 2011. Bio-management of root-knot disease of chick pea caused by Meloidogyne incognita. Indian Council of Agricultural Research 19(1), 159-163.
Sharma, A., Haseeb, A and Abuzar, S., 2006. Screening of field pea (Pisum sativum) selections for their reactions to root-knot nematode (Meloidogyne incognita). Journal of Zhejiang Univristy Science B. 7(3), 209–214.
Sharma , S., Singh, J., Munshi, GD. and Munshi, SK., 2010. Biochemical changes associated with application of biocontrol agents on Indian mustard leaves from plants infected with Alternaria blight. Archives of Phytopathology and Plant Protection 43 (4), 315-323.
Siddiqui, IA. and Mahmood, I., 1999. Role of bacteria in the management of plant parasitic nematodes: a review. Bioresource technology 69, 167–179.
Snedecor, GW. and Cochran, WG., 1980. Statistical Methods. 5th ed. Ames, IA: Iowa State Univ. Press; p. 593.
Snell, FD., Snell, CT., 1953. Colorimetric method. Vol. III, Van Nostrand Company, London, p.606.
Taylor, A L. and Sasser, J N., 1978. Biology, identification and control of root-knot nematodes (Meloidogyne species). IMP. Raleigh, N.C.: North Carolina State University Graphics; p. 111.
Youssef, MMA., El-Nagdi, Wafaa MA and Dawood, Mona G., 2018. Effect of sugar beet plant residues on population density of root knot nematode, Meloidogyne incognita infecting cowpea and biochemical changes in treated plants. Pakistan Journal of Nematology 36 (1), 41-48.
Zheng, Z., Zheng J., Zhang Z., Peng D. and Sun M., 2016. Nematicidal spore-forming Bacilli share similar virulence factors and mechanisms .Scientific Reports, 6 (31341); Doi: 10.1038/srep31341