Nematicidal Effects of Bacillus subtilis and Bacillus pumilus Against Meloidogyne incognita infecting Pea

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Wafaa Mohamed El-Nagdi H. Abd-El-Khair Mona G. Dawood


Bacillus subtilis (BS) and B. pumilus (BP1 and BP2) were applied alone as well as in combination for controlling Meloidogyne incognita infecting pea in pot experiment. Single treatments of bacteria significantly reduced the numbers of juvenile (J2) in soil (50-80%) ,J2 in roots (57-78%), females (55-74%), galls  (65-74%) and egg-masses (56-70%) , while the combination treatments of them significantly reduced the same parameters in the ranges of 29-76% , 61-77% , 46-69% , 47-67% and 40-61% , compared to 71 , 70 , 70 ,68 and 65% , with Carbofuran 10%, respectively.  Results showed that BP2 highly reduced the J2 in soil , BS highly reduced J2 in roots BS and BP1 highly reduced the numbers of females, galls and egg-masses in roots. On the other hand, BP1 + BP2 highly reduced the numbers of J2 in soil, J2, females and egg-masses in roots, while BS+BP1+BP2 treatment highly reduced the galls number.  B. subtilis and B. pumilus, as single treatment, in some parameters, had more nematicidal activity against M. incognita, than in combination treatments. Treatments also significantly increased the growth parameters of pea plants shoot length, shoot fresh and dry weight, leaves numbers and pod fresh and dry weight. The treatments improved the soluble protein and total phenolic compounds in treated pea plants.  


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El-Nagdi, W. M., Abd-El-Khair, H., & Dawood, M. G. (2018). Nematicidal Effects of Bacillus subtilis and Bacillus pumilus Against Meloidogyne incognita infecting Pea. Advances in Agricultural Science, 6(4), 52-59. Retrieved from


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