Effect of biofumigation and soil solarization on stem canker and black scurf diseases of potato (Solanum tuberosum L.) caused by Rhizoctonia solani PR2 isolate

Main Article Content

M. Tanbir Rubayet M. Khurshed Alam Bhuiyan Rayhanur Jannat M. Mahidul Islam Masum M. Motaher Hossain


An experiment was conducted to evaluate the effect of biofumigation and soil solarization on stem canker and black scurf diseases of potato caused by Rhizoctonia solani PR2 isolate. Before setting the field experiment, a series of in-vitro and in-vivo trails were laid out to select a virulent isolate PR2 of R. solani against the susceptible potato variety of cardinal and evaluated the Brassica spp. namely cabbage, cauliflower, mustard, and broccoli leaf extracts to select the best biofumigant against R. solani PR2 isolate in in-vitro condition. Among the different Brassica spp., Brassica nigra (mustard) was the most effective in inhibiting the radial growth (79.63%) of R. solani PR2 at 40% level of concentration followed by broccoli leaf extract (66.67%). At the field trials, in T5 treatment where used biofumigation and soil solarization was appeared to be most superior in reducing the pre and post emergence mortality of potato seedling. The lowest disease incidence (24.44% and 30.67%), and PDI (20.37% and 13.89%) also were found in stem canker and black scurf diseases, respectively at treatment T5 followed by T3 (where used biofumigation without soil solarization) treatment. The yield of potato 117.13% was increased in T5 treatment followed by T3 treatment (73.01%). The increase of yield not only because of the reduction of diseases but also it might be due to supplement of the organic materials in the soil as a result increases the amount of soluble nutrients and secret the growth promoting substances by beneficial soil microorganisms.


Download data is not yet available.

Article Details

How to Cite
Rubayet, M. T., Alam Bhuiyan, M. K., Jannat, R., Islam Masum, M. M., & Hossain, M. M. (2018). Effect of biofumigation and soil solarization on stem canker and black scurf diseases of potato (Solanum tuberosum L.) caused by Rhizoctonia solani PR2 isolate. Advances in Agricultural Science, 6(3), 33-48. Retrieved from http://aaasjournal.org/submission/index.php/aaas/article/view/80


Abdel-Rahim, M.F., Satour, M.M., Mickail, K.Y., El-Eraki, S.A., Grinstein, A., Chen, Y., Katan, J., 1988. Effectiveness of soil solarization in furrow-irrigated Egyptian soils. Plant Dis. 72, 143-146.
Adandonon, A., Aveling, T.A.S., Labuschagne, N., Tamo M., 2006. Biocontrol agents in combination with Moringa oleifera extract for integrated control of Sclerotium-caused cowpea damping-off and stem rot. Europ. J. Plant Pathol. 115, 409-418.
Akter, W., Bhuiyan, M.K.A., Sultana, F., Hossain, M.M., 2015. Integrated effect of microbial antagonist, organic amendment and fungicide in controlling seedling mortality (Rhizoctonia solani) and improving yield in pea (Pisum sativum L.). C. R. Biol. 338, 21-28.
Altomare, C., Norvell, W.A., Bjorkmanand, T., Harman, G.C., 1999. Solubilization of phosphates and micronutrients by the plant-growth promoting and biocontrol fungus Trichoderma harzianum Rifai. ?Appl. Environ. Microbiol. 65, 2926-2933.
Al-Turkib, A.I., Dick, W.A., 2003. Myrosinase Activity in Soil. Soil Sci. Soc. Am. J. 67, 139-145.
Andréasson, E., Bolt J.L., Höglund, A.S., Rask, L., Meijer, J., 2001. Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus. Plant Physiol. 127, 1750-1763.
Anonymous, 2016. Statistical Year Book of Bangladesh (2014-2015). Bangladesh Statistics Division, Ministry of Planning, Govt. of the People’s Republic of Bangladesh. pp. 318-319.
Aparna, K.P., Girija, V.K., 2018. Efficacy of Plant Oils and Oil Cakes against Rhizoctonia solani Kuhn Causing Collar Rot and Web Blight of Cowpea under in-vitro Conditions. Int. J. Curr. Microbiol. App. Sci. 7, 2091-2098.
Arora, R.K., Jyosana, S., 2007. Eco-friendly alternatives to pesticides in management of soil and tuber borne diseases of potato in organic farming. In: Sustainable Environmental Management: Dr Jayashree Deshpande Festschrift Volume (eds. L.V. Gangawane and V.C. Khilare). Daya Publishing House, New Delhi. pp. 1-6.
Barneet, H.L., Hunter, B.B., 1972. Illustrated genera of Imperfect fungi, forth ed., USA: Burges s Publishing Company.
Bhuiyan, K.A., Sen, B.C., 2013. Integrated management of Southern Blight disease of Carrot caused by Sclerotium rolfsii. Acta. Phytopathol. Sin. 43, 515-523.
Brammer, H., 1971. Soil Resources, Soil Survey Project, Bangladesh. AGL: SF Pck. 6. Technical Report. Dhaka, Bangladesh. pp. 3-8.
Brewer, M., Larkin, R.P., 2005. Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato. Crop Prot. 24, 939–950.
Butler, E.E., Bracker, C., 1970. Morphology and cytology of Rhizoctonia solani, in: J.R. Parmeter, Jr. (Ed.), Rhizoctonia solani Biology and Pathology, University of California Press, Berkeley, USA.
Carling, D.E., Kuninaga, S., Brainard, K.A., 2002. Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology. 92, 43-50.
Carling, D.E., Leiner, R.H., Westphale, P.C., 1989. Symptoms, signs and yield reduction associated with Rhizoctonia disease of potato induced by tuber borne inoculum of Rhizoctonia solani AG3. Am. Potato J. 66, 693-701.
Chandel, S., Sharma, S., 2014. Botanicals, biofumigants and antagonists application in managing stem rot disease caused by Rhizoctonia solani Kuhn in carnation. J. Bio. Pest. 7, 3-10.
Charron, C.S., Sams, C.E., 1999. Inhibition of Pythium ultimum and Rhizoctonia solani by shredded leaves of Brassica species. J. Am. Soc. Hort. Sci. 124, 462-467.
Chellemi, D.O., Olson S.M., Mitchell, D.J., 1994. Effects of soil solarization and fumigation on survival of soil-borne pathogens of tomato in northern Florida. Plant Dis. 78, 1167-1172.
Das, S., Shah, F.A., Butler, R.E., Falloon, R.E., Stewart, A., Raikar, S., Pitman, A.R., 2014. Genetic variability and pathogenicity of Rhizoctonia solani associated with black scurf of potato in New Zealand. Plant Pathol. 63, 651-666.
Dey, T.K., 2010. Tuber crops diseases-their identification, method of recording, rating scale and grading system. Oilseed Res. Center, BARI. Bangladesh. pp. 28-31.
Dey, T.K., Ali, M.S., 1994. Pathological research on tuber crops in Bangladesh. In: Proc. of workshop on Transf. of Tech. of CDP crops under Res. Extu. Linkage Program. held on Oct. 22-27, BARI. pp. 159-165.
Dubey, S.C., Tripathi, A., Upadhyay, BK, Deka, U.K., 2014. Diversity of Rhizoctonia solani associated with pulse crops in different agro-ecological regions of India. World J. Microbiol. Biotechnol. 30, 1699-1715.
Dwivedi, SK., Prasad, G., 2016. Integrated management of Sclerotium rolfsii: an overview. EJBPS, 3, 137-146.
El Balkali, A.M., Martin, M.P., 2006. Black scurf of potato. Mycologist. 20, 130–132.
Elmore, C.L., Stapelton, J.J., Bell, C.E., Devay, J.E., 1997. Soil solarization: A non-pesticidal method for controlling diseases, nematodes and weeds, UC DANR Pub. 21377 Oakland. pp. 10-14.
Faruk, M.I., Rahman, M.L., 2015. Management of Tomato Seedling Rot disease (Rhizoctonia solani) in Seedbed with Trichoderma harzianum bio-fungicide. Inter. Res. J. 5, 14-20.
Fayzalla, E.A., El-Barougy, E., ElRayes, M.M., 2009. Control of Soil Borne Pathogenic Fungi of Soybean by Biofumigation with Mustard Seed Meal. J. Appl. Sci. 9, 2272-2279.
Gomez, K.A., Gomez, A.A., 1984. Statistical Procedures for Agricultural Research. Second ed. NY, USA: John, Wiley and Sons.
Goswami, B.K., Bhuiyan, K.A., Mian, I.H., 2010. Morphological and pathogenic variations in the isolates of Rhizoctonia Solani in Bangladesh, Bangladesh J. Agril. Res. 35, 375-380.
Harman, G.E., 2000. Myths and dogmas of bio-control: Changes in perceptions derived from research on Trichoderma harzianumT-22. Plant Dis. 84, 377-393.
Harvey, S.G., Hannahan, H.N., Sams, C.E., 2002. Indian mustard and alkyl isothiocyanate inhibit Sclerotium rolfsii. J. Amer. Soc. Hort. Sci. pp. 127.
Hossain, M., Dey, T.K., Akther, S., Bhuiyn, M.K.R., Hoque, M.A., Kundu, B.C., Hossain M.A., Begum, S.N., 2008. Activities and achievements of Tuber Crops Research Centre at a Glance. Bulletin published by the Tuber Crops Research Centre, BARI, Gazipur. pp. 22-23.
Hyakumachi, M., 1994. Plant growth promoting fungi from Trufgrass rhizosphere with potential for disease suppression. Soil Organisms, 44, 33-38.
Kapoor, R.T., 2013. Soil Solarization: Eco-friendly Technology for Farmers in Agriculture for Pest Management. 2nd International Conference on Advances in Biological and Pharmaceutical Sciences (ICABPS'2013) Sept 17-18, Hong Kong. pp. 14-16.
Kirkegaard, J.A., Sarwar, M., 1998. Biofumigation potential of Brassicas. Plant and Soil. 201, 71-89.
Kirkegaard, J.A., Wong, P.T.W., Desmarchelier, J.M., 1996. In-vitro suppression of fungal root pathogens of cereals by Brassica tissues. Plant Pathol. 45, 593-603.
Kumar, M., Singh, J.K., Kumar, S., Kumar, A., 2017a. A Comprehensive Overview on Black Scurf of Potato. Int. J. Curr. Microbiol. App. Sci. 6, 4981-4994.
Kumar, V., Ankush, Tehlan, S.K., Kumar, A., Priyanka., 2017b. Soil solarization: an approach towards sustainable Agriculture. Innovative Farming. 2, 126-130.
Larkin, R.P., 2008. Relative effects of biological amendments and crop rotations on soil microbial communities and soil-borne diseases of potato. Soil Biol. Biochem. 40, 1341-1351.
Larkin, R.P., Griffin, T.S., 2007. Control of soil-borne potato diseases using Brassica green manures. Crop protection, 26, 1067-1077.
Matthiessen, J.N., Shackleton, M.A., 2005. Biofumigation: environmental impacts on the biological activity of diverse pure and plant-derived isothiocyanates. Pest Manag Sci. 61, 1043-1051.
Mian, I.H., 1995. Methods in plant pathology. IPSA-JICA project publication number twenty-four. Institute of Post Graduate Studies in Agriculture, Bangladesh. pp. 51-52.
Mithen, R.F., Lewis, B.G., Fenwick, G.R., 1986. In-vitro activity of glucosinolates and their products against Leptosphaeria maculans. Trans. Br. Mycol. Soc. 87, 433-440.
Morra, M.J., Kirkegaard, J.A., 2002. Isothiocyanate release from soil-incorporated brassica tissues. Soil Biol. Biochem. 34, 1683-1690.
Nene, Y.L., Thapliyal, P.N., 1979. Fungicides in Plant Disease Control, second Ed. New Delhi, India: Oxford and IBH publication Company.
Rahman, M.M., Ali, M.A., Ahmad, M.U., Dey, T.K., 2013. Effect of tuber-borne inoculum of Rhizoctonia solani on the development of stem canker and black scurf of potato. Bangladesh J. Plant Pathol. 29, 29-32.
Rahman, Z., Mafuzul Haque, A.H.M., Zaman, M.A., Amin, M.F., Das, A.K., 2012. Efficacy of two fungicides and two botanicals to control foot and root rot disease (Sclerotium rolfsii) of cowpea. Bangladesh J. Plant Pathol. 28, 29-32.
Razaq, M., Rab, A., Alam, H., Salahuddin, Saud, S., Ahmad, Z., 2015. Effect of Potash Levels and Plant Density on Potato Yield. J. Biol. Agric. Healthc. 5, 55-62.
Sen, B.C. 2010. Management of Carrot Diseases Caused by Rhizoctonia solani and Sclerotium rolfsii, (PhD thesis), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.
Shaban, W.I., El-Barougy, E., Zian, A.H., 2011. Control of lupine Fusarium wilt by biofumigation with mustard and canola seed meal. Tunisian J. Plant Prote. 6, 87-98.
Shaheed, S.M., 1984. Soil of Bangladesh: General Soil Types. Soil Resources Development Institute (SRDI) Dhaka, Bangladesh. 3p.
Smolinska, U., Morra, M.J., Knudsen, G.R., James, R.L., 2003. Isothiocyanates produced by Brassicaceae species as inhibitors of Fusarium oxysporum. Plant Dis. 87: 407–412.
Stapleton, J.J., DeVay, J.E., 1986. Soil solarization: A non-chemical approach for management of plant pathogens and pests. Crop Prote. 5, 190-198.
Sundar, A.R., Das, N.D., Krishnaveni, D., 1995. In-vitro Antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian J. Plant Protec. 23, 152-155.
Tsror, L., 2010. Biology, epidemiology, and management of Rhizoctonia solani on potato. J. Phytopathol. 158, 649-658.
Ui, T., 1973. Zonation in cultures Rhizoctonia solani Kuhn under continuous darkness. Trans. Mycol. Soc. Japan. 14, 179-184.
Wilson, S.P., Ahvenniemi, M.P., Lehtonen, J.M., Kukkonen, M., Rita, H., Valkonen, P.T.J., 2008. Biological and chemical control and their combined use to control different stages of the Rhizoctonia disease complex on potato through the growing season. Ann. Appl. Biol. 153, 307–320.
Zaman, S.M.H., Rahim, K., Howlader, M., 1982. Simple lessons from biometry, BARI. Bangladesh. pp. 24-54.
Zhang, X.Y., Yu, X.X., Yu, Z., Xue, Y.F., Qi, L.P., 2014. A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani. Breed Sci. 64, 156–163.