Exogenous application of salicylic acid improves tolerance of wheat plants to lead stress Salicylic acid improves tolerance of wheat plants to lead stress

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Saud Ali Dayl Alamri Manzer H Siddiqui Mutahhar Yahya Al-Khaishany Hayssam Mohamed Ali Abdullah Al-Amri Hala Khalid AlRabiah


Salicylic acid (SA) acts as a signaling molecule and plays an important role in various physiological and biochemical processes in plants. The aim of the present study was to evaluate the role of SA in the enhancement of lead (Pb) tolerance in wheat (Triticum aestivum) plants. When 2–3 true leaves had appeared, treatments were applied to the plants. The treatments were as follows: (i) no addition of SA and Pb (control), (ii) 2 µM SA + 0 mM Pb, (iii) 8 µM SA + 0 mM Pb, (iv) 0 mM SA + 2 mM Pb, (v) 2 µM SA + 2 mM Pb, and (vi) 8 µM SA + 2 mM Pb. One-way analysis of variances (ANOVA) was used to compare the means, and Duncan’s multiple-range test (DMRT) was used to determine significant (P < 0.05) differences among the individual means of treatments. Exposure of Pb severely affected wheat plants by reducing plant height, fresh and dry weight, photosynthetic pigments (Chl a and b, Chl a:b) and carbonic anhydrase enzyme activity, and by enhancing Chl degradation, electrolyte leakage (EL), malondialdehyde accumulation. Also, Pb treatment increased the accumulations of proline and total soluble carbohydrates (TSC) and activities of antioxidant enzymes [superoxide dismustase (SOD), catalase (CAT), and peroxidase (POD)]. However, application of SA induced biosynthesis of pigments by suppressing Chl degradation, and EL and malondialdehyde accumulation. Furthermore, SA treatments further enhanced the production of proline and TSC, and the activities of SOD, CAT, and POD. SA directly or indirectly improved physiological processes, which helped wheat plants to overcome the oxidative damage induced by Pb toxicity. Also, this study reveals that exogenous application of SA is beneficial for plant growth and development of wheat plants by suppressing ill effects of heavy metal stress. Therefore, this study opens up the hidden role of SA in tolerance of plants to heavy metal toxicity to explore its new regulatory role and defensive mechanism at physiological and molecular levels. Also, exogenous application of SA could be beneficial for sustainable agriculture.


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Alamri, S. A. D., Siddiqui, M., Al-Khaishany, M. Y., Ali, H. M., Al-Amri, A., & AlRabiah, H. K. (2018). Exogenous application of salicylic acid improves tolerance of wheat plants to lead stress. Advances in Agricultural Science, 6(2), 25-35. Retrieved from http://aaasjournal.org/submission/index.php/aaas/article/view/57


Aebi, H., 1984. Catalase in vitro. Methods in Enzymology, 105: pp.121–126.
Agami, R.A., Mohamed G.F., 2013. Exogenous treatment with indole-3-acetic acid and salicylic acid alleviates cadmium toxicity in wheat seedlings. Ecotoxicology and Environmental Safety, 94: pp.164–171.
Al-Whaibi, M.H., Siddiqui, M.H., Basalah, M.O., 2012. Salicylic acid and calcium-induced protection of wheat against salinity. Protoplasma, 249: pp.769–778.
ATSDR (Agency for Toxic Substances and Disease Registry), 2005. Toxicological Profile for Lead. Agency for Toxic Substances and Disease Registry. Georgia, pp. 1–577.
Baghai, N., Setia, R.C., Setia, N., 2002. Effects of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brassica napus L. (cv. GSL-1). Phytomorphology, 52: pp. 83–87.
Barnes, J.D., Balaguer, L., Manrique, E., Elvira, S., Davison, A.W., 1992. A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environmental and Experimental Botany, 32: pp. 85–100.
Bates, L.S., Waldren, R.P., Teare, I.D., 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: pp.205–207.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: pp. 248–254.
Chance, B., Maehly, A.C., 1955. Assay of catalase and peroxidases. Methods in Enzymology, 2: pp.764–775.
Dubois, N., Gilles. K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: pp.350-356.
Dugar, D., Bafna, A., (2013). Effect of Lead Stress on Chlorophyll Content, Malondialdehyde and peroxidase activity in Seedlings of mung bean (Vigna radiate). International Journal of Research in Chemistry and Environment, India, 3: pp. 20-25.
Dwivedi, R.S., Randhawa, N.S., (1974). Evaluation of rapid test for hidden hunger of zinc in plants. Plant and Soil, Netherlands, 40: pp. 445–451.
Fan, T., Yang, L., Wu, X., Ni, J., Jiang, H., Zhang, Q., Fang, L., Sheng, Y., Ren, Y., Cao, S., 2016. The PSE1 gene modulates lead tolerance in Arabidopsis. Journal of Experimental Botany, 67: pp.4685-4695.
Foyer, C.H., Halliwell, B., 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta, 133: pp.1–25.
Giannopolitis, C.N., Ries, S.K., 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiology, 59: pp. 309–314.
Gupta, D.K., Huang, H.G., Corpas F.J., 2013. Lead tolerance in plants: strategies for phytoremediation. Environmental Science and Pollution Research, 20: pp. 2150–2161.
Heath, R.L., Packer, L., 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: pp.189–198.
Klessig D.F., Malamy, J., 1994. The salicylic acid signal in plants. Plant Molecular Biology, 26: pp.1439-1458.
Kovács, V., Gondor, O.K., Szalai, G., Darkó, E., Majláth, I., Janda, T., Pál, M. (2014). Synthesis and role of salicylic acid in wheat varieties with different levels of cadmium tolerance. Journal of Hazardous Materials, 280: 12-19.
Leal-Alvarado, D.A., Espadas-Gil, F., Sáenz-Carbonell, L., Talavera-May, C., Santamaría, J.M., 2016. Lead accumulation reduces photosynthesis in the leadhyper-accumulator Salvinia minima Baker by affecting the cellmembrane and inducing stomatal closure. Aquatic Toxicology, 171: pp.37–47.
Lee, H.I., León, J., Raskin, I., 1995. Biosynthesis and metabolism of salicylic acid. Proceedings of the National Academy of Sciences of the United States of America, 92: pp. 4076-4079.
Liu, Z., Ding, Y., Wang, F., Ye, Y., Zhu, C., 2016. Role of salicylic acid in resistance to cadmium stress in plants. Plant Cell Reports, 35: pp. 719–731.
Lutts, S., Kinet, J.M., Bouharmont, J., 1995. Changes in plant response to NaCl during development of rice (Oryza sativa L) varieties differing in salinity resistance. Journal of Experimental Botany, 46: pp.1843–1852.
Mehta, S.K., Gaur, J.P., 1999. Heavy-metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris. New Phytologist, 143: pp. 253–259.
Misra, N., Saxena, P. 2009. Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science, 177: pp. 81–189.
Mourato, M.P., Moreira, I.N., Leitão, I., Pinto, F.R., Sales, J.R., Martins, L.L., 2015. Effect of heavy metals in plants of the genus Brassica. International Journal of Molecular Sciences, 16: pp. 17975–17998.
Nareshkumar, A., Nagamallaiah, G.V., Pandurangaiah, M., Kiranmai, K., Amaranathareddy, V., Lokesh, U., Venkatesh, B., Sudhakar, C., 2015. Pb-stress induced oxidative stress caused alterations in antioxidant efficacy in two groundnut (Arachis hypogaea l.) cultivars. Agricultural Sciences, 6: pp. 1283-1297.
Rahmani, I., Ahmadi. N., Ghanati, F., Sadeghi, M., 2015. Effects of salicylic acid applied pre- or post-transport on post-harvest characteristics and antioxidant enzyme activity of gladiolus cut flower spikes. New Zealand Journal of Crop and Horticultural Science, 43: pp. 294–305.
Rivas-San Vicente, M., Plasencia, J. 2011. Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany, 62: 3321-3338.
Ronen, R., Galun, M., 1984. Pigment extraction from lichens with dimethyl sulfoxide (DMSO) and estimation of chlorophyll degradation. Environmental and Experimental Botany, 24: pp. 239-245.
Rosa, M., Prado, C., Podazza, G., Interdonato, R., González, J.A., Hilal, M., Prado, F.E., 2009. Soluble sugars—metabolism, sensing and abiotic stress: A complex network in the life of plants. Plant Signaling Behavior, 4: pp. 388–393.
Ruley A.T., Sharma, N.C., Sahi, S.V., 2004. Antioxidant defense in a lead accumulating plant, Sesbania drummondii. Plant Physiology and Biochemistry, 42: pp. 899–906.
Sahu, G.K., Kar, M., Sabat, S.C., 2002. Electron transport activities of isolated thylakoids from wheat plants grown in salicylic acid. Plant Biology, 4: pp. 321-328.
Shakirova, F.M., Bezrukova, M.V., Yuldashev, R.A., Fatkhutdinova, R.A., Murzabaev, A.R. (2012). Involvement of lectin in the salicylic acid–induced wheat tolerance to cadmium and the role of endogenous ABA in the regulation of its level. Doklady Biological Sciences, 448: 49-51.
Sharma, P., Dubey, R.S., 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17: pp. 35-52.
Shitov, A.V., Terentyev, V.V., Zharmukhamedov, S.K., Rodionova, M.V., Karacan, M., Karacan, N., Klimov V.V., Allakhverdiev, S.I., 2018. Is carbonic anhydrase activity of photosystem II required for its maximum electron transport rate?. Biochimica et Biophysica Acta (BBA) – Bioenergetics, 1859: 292-299.
Siddiqui, M.H., Al-Whaibi M.H., Sakran A.M., Basalah, M.O., Ali, H.M., 2012. Effect of calcium and potassium on antioxidant system of Vicia faba L. under cadmium stress. International Journal of Molecular Sciences, 13: pp. 6604-6619.
Siddiqui, M.H., Al-Whaibi, M.H., Ali, H.M., Sakran, A.M., Basalah, M.O., Al-Khaishany, M.Y.Y., 2013. Mitigation of nickel stress by the exogenous application of salicylic acid and nitric oxide in wheat. Australian Journal of Crop Science, 7: 1780-1788.
Siddiqui, M.H., Alamri, S.A., Al-Khaishany, M.Y.Y., Al-Qutami M.A., Ali, H.M., Al-Whaibi, M.H., Al-Wahibi, M.S., Alharby H.F., 2016. Mitigation of adverse effects of heat stress on Vicia faba by exogenous application of magnesium. Saudi Journal of Biological Sciences, http://dx.doi.org/10.1016/j.sjbs.2016.09.022.
Siddiqui, M.H., Mohammad, F., Khan, M.N., 2009. Morphological and physio-biochemical characterization of Brassica juncea L. Czern. & Coss. genotypes under salt stress. Journal of Plant Interactions, 4: pp. 67-80.
Smith, G.S., Johnston, C.M., Cornforth, I.S. (1983). Comparison of nutrient solutions for growth of plants in sand culture. New Phytologist, 94: pp. 537–548.
Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K., Sutton, D.J. (2012). Heavy metals toxicity and the environment. In: Luch A, editor. Molecular, Clinical and Environmental Toxicology: Springer Basel; p. 133-64.
Venkatesh, J., Park. S.W., 2014. Role of L-ascorbate in alleviating abiotic stresses in crop plants. Botanical Studies, 55: p.38.
Zengin, F.K., Munzuroglu, O. 2005. Effects of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biologica Cracoviensia Series Botanica, 47: pp. 157–164.