Spectral and SPAD Chlorophyll-meter Determination of some Photosynthetic Pigments of Chard and Radish

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Abstract

            In this study efficacy of three different commonly used solvents viz. acetone, methanol, and diethyl ether with three concentrations (70, 80 and 99%) were used for spectrophotometrically extraction of chlorophyll-a, chlorophyll-b and total carotenoids from chard (Beta vulgaris L.) and radish (Raphanus sativus L.) leaves, with comparing the results with values of SPAD-chlorophyll meter device. The results revealed that the best extraction solvent for simultaneous determination of photosynthetic pigments for chard plant was diethyl ether at 70, 80 and 70% for chlorophyll-a and b and total carotenoids respectively, whereas for radish plant the 99% acetone  was the best solvent for simultaneous determination of chlorophyll-a and total carotenoids, while for chlorophyll-b it was 99% diethyl ether. Furthermore, the study indicated non-significant relationship between spectrophotometrically estimated pigments and SPAD-chlorophyll meter values for chard and radish leaves.

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