Effects of 3-methylthiopropionic acid (MTPA) phytotoxin produced by Rhizoctonia solani on reactive oxygen species (ROS) metabolism of potato plants

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Frederick Kankam Pu Lumei Qiu Huizhen


Studies were conducted to investigate the effects of 3-methylthiopropionic acid (MTPA) produced by Rhizoctonia solani on antioxidant enzyme activities of potato in causing stem canker. The experiments were laid out in a completely randomized design with six treatments replicated five times. The treatments were 1, 2, 4, and 8 mM concentrations of MTPA while sterilized soil only (CK1) and sterilized soil with R. solani inoculum (CK2) served as control treatments. Results showed that compared with control plants (CK1), the activities of enzyme antioxidants such as superoxide dismutase (SOD), peroxidases (POD), and catalase (CAT) in potato stems treated with 8 mM MTPA increase by 86.95, 203.64, and 123.28%, respectively. Similarly, the activity of chitinase in the roots, stems and that of leaves increases by 120.22, 145.45, and 143.91%, respectively compared to CK1. However, the levels of malondiadehyde (MDA) in the roots, stems and leaves of 8 mM MTPA-treated plants decreased by 167.80, 156.52, 158.30%, respectively, compared to CK1. The study revealed that MTPA increased the activities of the anti-disease effective antioxidant enzymes of the potato plant due to the higher levels of ROS.  


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Kankam, F., Lumei, P., & Huizhen, Q. (2018). Effects of 3-methylthiopropionic acid (MTPA) phytotoxin produced by Rhizoctonia solani on reactive oxygen species (ROS) metabolism of potato plants. Advances in Agricultural Science, 7(1), 11-23. Retrieved from http://aaasjournal.org/submission/index.php/aaas/article/view/111
Author Biographies

Pu Lumei, College of Science, Institute of Agricultural Resources Chemistry and Application, Gansu Agricultural University, Lanzhou 730070, China

College of Science, Institute of Agricultural Resources Chemistry and Application,


Qiu Huizhen, Department of Agronomy, University for Development Studies, P.O. Box 1882, Tamale, Ghana

College of Resources and Environmental Sciences,



Ahvenniemi, A., Lehtonen, M., Wilson, P., Valkonen, J. 2007. Disease cycle of seed-borne Rhizoctonia-disease, new and old pathogens in changing climate. Abstracts from the Proceedings of the EAPR Pathology Section Seminar, Hattula, Finland, 2-6th July 2007: 26.
Arici, S. E., Sanli, A. 2014. Effect of some essential oils against Rhizoctonia solani and Streptomycetes scabies on potato plants in field conditions. Annual Research and Review in Biology 4(12): 2027?2036.
Atkinson, D., Thornton, M. K., Miller, J. S. 2010. Development of Rhizoctonia solani on stems, stolons and tubers of potatoes I. Effect of inoculum source. American Journal of Potato Research 87: 374?381.
Azevedo, H., Lino-Neto, T., Tavares, R. M. 2008. The necrotroph Botrytis cinerea induces a non-host type II resistance mechanism in Pinus pinaster suspension-cultured cells plant and cell physiology. Plant Cell Physiology 49(3): 386?395.
Bartz, F. E., Glassbrook, N. J., Danehower, D. A., Cubeta, M. A. 2012. Elucidating the role of phenylacetic acid metabolic complex in the pathogenic activity of Rhizoctonia solani anastomosis group 3. Mycologia 104: 793?803.
Chen, S., Ding, G., Wang, Z., Cai, H., Xu, F. 2015. Proteomic and comparative genomic analysis reveals adaptability of Brassica napus to phosphorus-deficient stress. Journal of Proteomics 117: 106?119.
Dou, J. H., Yu, S. X., Fan, S. L. 2010. SOD and plant stress resistance. Molecular Plant Breeding 8(2): 359?364.
Duffy, B., Schouten, A., Raaijmakers, J. M. 2003. Pathogen self-defense: mechanisms to counteract microbial antagonism. Annual Review of Phytopathology 41: 501?38.
Fink, W., Liefland, M., Mendgen, K. 1988. Chitinases and ?-1,3-glucanases in the apoplastic compartment of oat leaves (Avena sativa L.). Plant Physiology 88: 270?285.
Garcia-Limones, C., Hervas, A., Navas-Cortes, J. A., Jimenez-Diaz, R. M., Tena, M. 2002. Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp. ciceris. Physiology and Molecular Plant Pathology 61: 325?337.
Gkarmiri, K., Finlay, R. D., Alström, S., Thomas, E., Cubeta, M. A., Högberg, N. 2015. Transcriptomic changes in the plant pathogenic fungus Rhizoctonia solani AG-3 in response to the antagonistic bacteria Serratia proteamaculans and Serratia plymuthica. Genomics 16: 630.
Gommers, F. L., Bakker, J., Nymbrg, H. 1982. Dithiophenes as singlet oxygen sensitizers. Phytochemistry and Photobiology 35: 615?619.
Hartman, G. L., Huang, Y. H., Li, S. 2004. Phytotoxicity of Fusarium solani culture filtrates from soybeans and other hosts assayed by stem cuttings. Australasian Plant Pathology 33: 9?24.
Joseph, L. M., Tan, T. K., Wong, S. M. 1998. Antifungal effects of hydrogen peroxide and peroxidase on spore germination and mycelia grow of Pseudocercospora species. Canadian Journal of Botany 76(12): 2119?2124.
Kankam, F., Long, H., He, J., Zhang C., Zhang, H., Pu, L., Qiu, H. 2016. 3-Methylthiopropionic acid of Rhizoctonia solani AG-3 and its role in the pathogenicity of the fungus. The Plant Pathology Journal 32(2): 85?94.
Kankam, F., Pu, Qiu, H., Pu, L., Long, H., He, J., Zhang, C., Zhang, H. 2016b. Isolation, purification and characterization of phytotoxins produced by Rhizoctonia solani AG-3, the cause agent of potato stem canker. American Journal of Potato Research 93: 321?330.
Kavroulakisa, N., Ehaliotisa, C., Ntougiasa, S., Zervakisa, G. I., Papadopoulou, K. K. 2005. Local and systemic resistance against fungal pathogens of tomato plants elicited by compost derived from agricultural residues. Physiology and Molecular Plant Pathology 66: 163?174.
Kim, S. Y., Kim, J. Y., Kim, S. H. 2007. Surfactin from Bacillus subtilis displays antiproliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression. FEBS Letters 581(5): 865?871.
Lehtonen, M. J., Ahvenniemi, P., Wilson, P. S., German-Kinnari, M., Valkonen, J. P. T. 2008a. Biological diversity of Rhizoctonia solani (AG-3) in a northern potato cultivation environment in Finland. Plant Pathology 57(1): 141?151.
Lehtonen, M. J., Somervuo, P., Valkonen, J. P. T. 2008b. Infection with Rhizoctonia solani induces defense genes and systemic resistance in potato sprouts grown without light. Phytopathology 98(11): 1190?1198.
Manoranjan, K., Dinabandhu, M. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology 57: 315?319.
Mao, B., Song, W., Chen, S., Liu, X., Lai, Q., Li, D. 2012. Modification of membrane lipid peroxidation and antioxidant enzymes activation in transgenic rice resistant to Rhizoctonia solani. African Journal of Biotechnology 11(21): 4841?4148.
Nakazaki, T., Tsukiyama, T., Okumoto, Y., Kageyama, D., Naito, K., Inouye, K., Tanisaka, T. 2006. Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.). Genome 49(6): 619?630.
Oliveira, M. T., Medeiros, C. D., Frosi, G., Santos, M. G. 2014. Different mechanisms drive the performance of native and invasive woody species in response to leaf phosphorus supply during periods of drought stress and recovery. Plant Physiology and Biochemistry 82: 66?75.
Qiu, Z-B., Guo, J-L., Zhang, M-M., Lei, M-Y., Li, Z-L. 2013. Nitric oxide acts as a signal molecule in microwave pretreatment induced cadmium tolerance in wheat seedlings. Acta Physiologiae Plantarum 35: 65?73.
Schraudner, M., Ernst, D., Langebartels, C., Sandermann, H. 1992. Biochemical plant responses to ozone III. Activation of the defense-related proteins ?-1,3-glucanase and chitinase in tobacco leaves. Plant Physiology 99: 1321?1328.
Tsror, L., Peretz-Alon, I. 2005. The influence of the inoculum source of Rhizoctonia solani on development of black scurf on potato. Journal of Phytopathology 153: 240?244.
Woodhall, J. W., Lees, A. K., Edwards, S. G., Jenkinson, P. 2008. Infection of potato by Rhizoctonia solani: Effect of anastomosis group. Plant Pathology 57(5): 897?905.
Wu, H-S., Yang, X-N., Fan, J-Q., Miao W-G., Ling, N., Xu, Y-C., Huan, Q-W., Shen, Q. 2009. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms. BioControl 54: 287?300.