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Leaf area (LA) is a valuable key for plant physiological studies, therefore accurate and simple models for LA determination are important for many experimental comparisons. Field experiment was conducted at the Teaching and Research Farm of the Federal University of Agriculture, Abeokuta (07o15’N, 03o 25’E) in the forest-savannah transition zone of South West Nigeria in 2013 to estimate leaf area (LA) of peppermint (Mentha piperita L.) using functions between plant LA and fresh weight (FW), dry weights (DW) and leaf dimensions (width-W and length-L) to identify appropriate functions for use in models estimating leaf area of peppermint. Leaf samples were randomly selected from the lower, middle and upper parts of the plant at 30, 60, 90 and 120 days after transplanting (DAT). Leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights of 150 leaf samples were assessed and compared with actual leaf area measured by graph tracing method, to test their accuracy and reliability using Y = a + bX model. There was a highly significant correlation (r = 0.6 to 0.9) between actual leaf area and the corresponding leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights. Regression analyses of LA versus FW, DW, L, W, L2, W2 and the products of these dimension revealed several models that could be used for estimating the area of individual peppermint leaf. Among the models, one based on length dimension (LA = a + bL) r = 0.9, R2 = 0.96, RMSE = 0.03 was the most accurate. To validate this model, actual leaf area of 60 leaf samples obtained by graph tracing method was compared with leaf area estimated by the model at 30, 60, 90 and 120 DAS in another trial in 2014 wet season. The leaf area estimated by the models strongly agreed with the measured value of leaf area as evident from high value of R2 (0.99) and low RMSE (0.03). The validation of the models indicates that model (LA = a + bL) was accurate and reliable to determine the leaf area of peppermint and therefore would be very useful for field workers dealing with large samples.
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Beemnet, M. K., Solomon, A. J. Texenia, O. S. 2010. Agronomic characters, leaf and essential oil yield of peppermint (Mentha piperita) as affected by harvesting age and row spacing. Medicinal and Aromatic Plant Science and Biotechnology 5(1): 49-53.
Bhatt, M., Chanda, S.V., 2003. Prediction of leaf area in Phaseolus vulgaris by non-destructive method. Bulg. J. Plant Physiol. 29, 96-100.
Blanco, F.F., and Folegatti, M.V. 2005. Estimation of leaf area for green house cucumber by linear measurements under salinity and grafting. Scientia Agricola, 62:305-309. http://dx.doi.org/10.1590/ S0103-90162005000400001.
Blank, A.F., Carvalho Filho, J.L.S. de; Santos Neto, A.L. dos, Alves, P.B. ; Arrigoni-Blank, M.F.; Silvamann, R.; Mendonça, M.C. 2004. Caracterização morfológicae agronômica de acessos de manjericão e alfavaca. Horticultura Brasileira, Brasília, 22: 113-116.
Cho, Y.Y., Oh, S., Oh, M.M., Son, J.E., 2007. Estimation of individual leaf area, fresh weight, and dry weight of hydroponically grown cucumbers (Cucumis sativus L.) using leaf length, width, and SPAD value. Sci. Hort. 111, 330-334.
Cristofori V, Rouphael Y, Mendoza-de Gyves E and Bignami C. 2007. A simple model for estimating leaf area of hazelnut from linear measurements. Sci Hortic 113: 221-225.
Fallovo, C., Cristofori, V., Mendoza, E., Rivera, C. M., Rea, R., and Fanasca, S. 2008. Leaf area estimation model for small fruits from linear measurements. Horticultural Science, 43, 2267-2267.
Gamper, H., 2005. Nondestructive estimates of leaf area in white clover using predictive formulae: The contribution of genotype identity to trifoliate leaf area. Crop Sci. 45, 2552-2556.
Granier, C., Massonnet, C., Turc, O., Muller, B., Chenu, K., Tardieu, F., 2002. Individual leaf development in Arabidopsis thaliana: a stable thermal-time-based programme. Ann. Bot. 89, 595-604.
Joseph-Adekunle, T.T. and Daramola, O.S. (201/4). Influence of organic amendments on growth and yield of peppermint under tropical condition. Journal of Organic Agricuulture and Environment 2: 48-53.
Kandiannan, K., Parthasarathy, U., Krishnamurthy, K.S., Thankamani, C.K., Srinivasan, V., 2009. Modeling individual leaf area of ginger (Zingiber officinale Roscoe) using leaf length and width. Sci. Hort. 120, 532-537.
Lu, H.Y., Lu, C.T., Wei, M.L., Chan, L.F., 2004. Comparison of different models for nondestructive leaf area estimation in taro. Agron. J. 96: 448-453.
Ma, L., Gardner, F.P., Selamat, A., 1992. Estimation of leaf area from leaf and total mass measurements in peanut. Crop Sci. 32, 467-471.
Mousavi Bazaza A.,, Z. Karimian Farimana, M. and Bannayanb. 2011 Modeling individual leaf area of basil (Ocimum basilicum) using different methods. International Journal of Plant Production 5 (4):439-447
Montero, F.J., De Juan, J.A., Cuesta, A., Brasa, A., 2000. Nondestructive methods to estimate leaf area in Vitis vinifera L. Hort. Sci. 35(4): 696-698.
NeSmith, D. S. 1992. Estimating summer squash leaf area non-destructively. Hort Science 27(1): 77.
Potdar, M.V., Pawar, K.R., 1991. Non-destructive leaf area estimation in banana. Sci. Hort. 45, 251-254.
Pinto, A. C. R., Rodrigues, T. J. D., Barbosa, J. C. and Leite, I. C. 2004. Leaf area prediction models for Zinnia elegans Jack, Zinnia haageana and 'profusion cherry'. Science Agriculture (Piracicaba, Braz.) 61(1): 47-52.
Pouono, K., Kumar, D. R. and Lauckner, F. B. 1990. Determination of leaf area in cacao (Theobroma cacao L.). Tropical Agriculture 67(1): 82 - 84.
Raju, V. R., Radhakrishan, S., Venkataramanan D. and Krishnamurthy, W. R. 1991. Leaf area determination in Coffea arabica L. Journal of Coffee Research 21(2): 109 -117.
Rouphael Y, Mouneimne AH, Ismail A, Mendoza-de Gyves E, Rivera CM and Colla G. 2010. Modeling individual leaf area of rose (Rosa hybrida L.) based on leaf length and width measurement. Photosynthetica 48: 9-15.
Souza, M.C. 2015. Non-destructive model to estimate the leaf area of multiple Vochysiaceae species. Brazilian Journal of Botany, 38:.903-909. Available from:
Salau, A. W., Olasantan, F.O. 2004. Rapid leaf area Estimation of pumpkin (Cucurbita maxima). ASSET Series A. (2006) 6(1): 255-258
Uzun, S. and Celik, H. 1999. Leaf area prediction models (Uzcelik-I) for different horticultural plants. Tropical Journal of Agriculture and Forestry 23: 645 - 650.
Wink. M. 2003. Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective. Journal of Phyto chemistry 64: 3–19.