Rapid leaf area measurement methods for Peppermint (Mentha piperita L.) grown under tropical condition

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Samuel Olumide Daramola Faucett Olagundoye Olasantan Adewale Waheed Salau Patience Mojibade Olorunmaiye Joseph Aremu Adigun Tunrayo Tinuoye Joseph-Adekunle Omobolanle Adewale Osipitan


Leaf area (LA) is a valuable key for plant physiological studies, therefore accurate and simple models for LA determination are important for many experimental comparisons. Field experiment was conducted at the Teaching and Research Farm of the Federal University of Agriculture, Abeokuta (07o15’N, 03o 25’E) in the forest-savannah transition zone of South West Nigeria in 2013 to estimate leaf area (LA) of peppermint (Mentha piperita L.) using functions between plant LA and fresh weight (FW), dry weights (DW) and leaf dimensions (width-W and length-L) to identify appropriate functions for use in models estimating leaf area of peppermint. Leaf samples were randomly selected from the lower, middle and upper parts of the plant at 30, 60, 90 and 120 days after transplanting (DAT). Leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights of 150 leaf samples were assessed and compared with actual leaf area measured by graph tracing method, to test their accuracy and reliability using Y = a + bX model. There was a highly significant correlation (r = 0.6 to 0.9) between actual leaf area and the corresponding leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights. Regression analyses of LA versus FW, DW, L, W, L2, W2 and the products of these dimension revealed several models that could be used for estimating the area of individual peppermint leaf. Among the models, one based on length dimension (LA = a + bL) r = 0.9, R2 = 0.96, RMSE = 0.03 was the most accurate. To validate this model, actual leaf area of 60 leaf samples obtained by graph tracing method was compared with leaf area estimated by the model at 30, 60, 90 and 120 DAS in another trial in 2014 wet season. The leaf area estimated by the models strongly agreed with the measured value of leaf area as evident from high value of R2 (0.99) and low RMSE (0.03). The validation of the models indicates that model (LA = a + bL) was accurate and reliable to determine the leaf area of peppermint and therefore would be very useful for field workers dealing with large samples.  


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Daramola, S., Olasantan, F. O., Salau, A. W., Olorunmaiye, P. M., Adigun, J. A., Joseph-Adekunle, T. T., & Osipitan, O. A. (2018). Rapid leaf area measurement methods for Peppermint (Mentha piperita L.) grown under tropical condition. Advances in Agricultural Science, 6(3), 123-131. Retrieved from http://aaasjournal.org/submission/index.php/aaas/article/view/100


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