Advances in Applied Agricultural Sciences 2 (2014); 12: 11-26
Comparative Study of Mutagens Effectiveness for Elite Lines with Enhanced Thebaine in Commercial Poppy (Papaver somniferum L.)
Avijeet Chatterjee 1, Sudhir Shukla 2*, Anu Rastogi 2, Brij Kishore Mishra 2 and Sant Prasad Singh 2
1 Present address: Botany Dept., National Post Graduate College, Lucknow, India. 2 Division of Genetics and Plant Breeding, National Botanical Research Institute, Lucknow, India.
For the first time we made a comparison between two stable varieties NBRI-1 and NBRI-5 of opium poppy for their sensitivity against mutagens using physical (gamma rays), chemical (ethyl methyl sulphonate) and combined mutagenesis. It was noticed that NBRI-5 was a better genotype having maximum mutagenic effectiveness as well as efficiency and showed most of the desirable variations in positive direction with respect to NBRI-1. Ethyl methyl sulphonate and combined treatments in their higher doses were most effective mutagens incorporating maximum reduction in germination percentage. Combined doses were also proved to be most effective for causing sterility of pollens. The dose kR 40 and among all combined doses, dose kR10+0.4%EMS was found to be an appropriate dose showing positive shift for most of the desirable traits especially thebaine. Selection for ideal plant type having high thebaine up to 10.73% in comparison to the existing varieties having thebaine 1-2%, could be made.